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INFO | PROCEDURE
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Telomere length analysis utilizing multicolor flow-FISH can be performed on subsets of human leukocytes from peripheral blood collected from individuals, in this case a DIAGNOSTIC PROCEDURE for an 83 year old. To obtain the flow cytometric histograms for the auto-fluorescence (b) and telomere fluorescence of each subset of cells, the following gating strategy is used: 1. Control cells (R1 + R4), lymphocytes (R2 + R4) and granulocytes can be distinguished based on their staining with the DNA dye LDS751 and on forward scatter and light scatter properties 2. The green fluorescence of cells gated in (a) hybridized in the absence (b) or presence (c) of fluorescein-labeled PNA probe is shown relative to LDS751 fluorescence 3. Antibodies specific for CD45RA and CD20 cells are used (e) to perform telomere length analysis of specific populations within the lymphocyte gate (R2 + R4) 4. Telomere length measurements (g) for a single individual will vary for each cell type from one individual |
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